normal human intestinal epithelial cells  (ATCC)

Journal: European Journal of Medical Research

Article Title: A core stemness-associated module reveals PLK1, NUF2, KIF23, CDCA8, TOP2A, CENPF, AURKA, and ASPM as key genes in rectal cancer

doi: 10.1186/s40001-025-03649-2

Figure Lengend Snippet: Experimental validation of the eight-gene panel and PLK1 manipulation in colorectal cell models. A qRT–PCR of ASPM, AURKA, CDCA8, CENPF, KIF23, NUF2, PLK1, TOP2A in SW620, Caco-2, and HIEC-6. B qRT–PCR measurement of PLK1 after transfection with si-PLK1#1/#2 or siNC in SW620 and Caco-2; si-PLK1#1 used for subsequent assays. C Wound-healing assay (0 and 48 h) in SW620 and Caco-2; representative images and quantification of wound width. D Matrigel transwell invasion of SW620 and Caco-2 (48 h); representative micrographs and quantification (cells/field). E CCK-8 time-course curves at 0, 24, 48, 72 h for SW620 and Caco-2; OD 450 values plotted. All procedures were performed in triplicate ( n = 3). F Flow cytometry was used to assess the effect of PLK1 silencing on the apoptotic capacity of cancer cells. *Statistical significance is indicated as follows: * P < 0.05, *** P < 0.001, **** P < 0.0001

Article Snippet: Human normal intestinal epithelial cells HIEC-6 (CRL-3266, ATCC, Manassas, VA, USA) and human colorectal cancer cell lines SW620 (TCHu101, National Collection of Authenticated Cell Cultures/Shanghai Cell Bank, Shanghai, China) and Caco-2 (SCSP-5027, Shanghai Cell Bank, Shanghai, China) were used.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Transfection, Wound Healing Assay, CCK-8 Assay, Flow Cytometry